Detection of Bacterial Contamination in Dental Unit Water Lines and Testing the Effectiveness of Disinfectants against these Contaminants

Aim: Contaminated dental unit water lines (DUWLs) are a possible source for spreading microorganisms in practices. The aim of this study was to detect the bacterial contamination and investigate effectiveness disinfectants.
 Methodology: Bacterial detected by a) using culture heterotrophic bacteria, total coliforms Pseudomonas aeruginosa b) Scanning electron microscopy (SEM) DUWLs tube. Subsequently, dentists were suggested treat with disinfectants eradicate contaminants its tested after three months.
 Results: samples ranged from not 2.38×106 CFU/mL. Out 34 tested, 30 (88.24%) exceeded Centers Disease Control Prevention (CDC) Environmental Protection Agency (EPA) recommended threshold ≤500 CFU /mL, whereas only 4 (11.76%) met standards. Contamination P. 52.94% 64.7% respectively. SEM displayed dense biofilm on tubing confirming contamination. intervention disinfection resulted more than 50% acceptable count test performed Conclusion: high rate highlights need disinfect monitor quality periodically.